一、个人简介

副教授，硕士生导师。2014年获得博士学位，博士期间从事基因治疗相关研究工作；2017-2021年在美国从事3年多的博士后工作，博士后期间针对神经系统自免疫疾病的发病机制和治疗开展研究。在国际顶级期刊STTT, Sci Immunol, JCI, Sci Trans Med, PNAS, Cell Mol Immunol, Front Immunol等发表论文20多篇，累计影响因子超过200；授权专利9项；主持或参与国家自然科学基金、陕西省自然科学基金、中央高校基金等科研项目14项；担任International Journal of Immunology编委；Molecular Therapy-Nucleic Acids、International Journal of Biological Sciences、Molecular Therapy - Methods & Clinical Development、Molecular Neurobiology等国际知名杂志的审稿人。

二、主要研究领域及兴趣

我国“十四五”规划和2035年远景目标刚要中，“脑科学”正式被列为八大战略性基础核心领域之一。本实验室的研究主要聚焦于神经系统炎症相关疾病的发病机制和治疗方法、脑胶质瘤的免疫逃逸机制和免疫治疗。希望通过对影响人类健康的中枢神经系统重大疾病发病机制的探索，发掘新的治疗手段，并实现向临床应用的转化。

三、代表性论文

1.Zhang W#, Xiao D#, Mao Q, Xia H. (2023) Role of neuroinflammation in neurodegeneration development.STTT, 8(1):267, IF=39.3

2. Zhang, W#., Xiao, D#., Li, X., Zhang, Y., Rasouli, J., Casella, G., Boehm, A., Hwang, D., Ishikaw, L.L.W., Thome, R., Ciric, B., Curtis, M., Rostami, A., Zhang, G. X. (2022) SIRT1 inactivation switches reactive astrocytes to an anti-inflammatory phenotype in CNS autoimmunity. JCI, doi:10.1172/JCI151803, IF=19.46

3. Rasouli, J., Casella, G., Zhang, W., Xiao D., Kumar, G., Fortina, P., Zhang, G-X., Ciric, B., Rostami, A. (2022) Transcription factor RUNX3 mediates plasticity of ThGM cells toward Th1 phenotype. Front Immunol, 3355, IF=7.97

4. Hwang, D., Seyedsadr, M. S., Ishikawa, L. L. W., Boehm, A., Sahin, Z., Casella, G., Jang, S., Gonzalez, M. V., Garifallou, J. P., Hakonarson, H., Zhang, W., Xiao, D., Rostami, A., Zhang, G. X., Ciric, B. (2022) CSF-1 maintains pathogenic but not homeostatic myeloid cells in the central nervous system during autoimmune neuroinflammation. Proc Natl Acad Sci U S A, 119, e2111804119. IF=12.78

5. Xiao D#, Zhang W#, Wang Q, Li X, Zhang Y, Rasouli J, Casella G, Ciric B, Curtis M, Rostami A, Zhang G-X. (2021) CRISPR-mediated rapid generation of neural cell-specific knockout mice facilitates research in neurophysiology and pathology. Molecular Therapy - Methods & Clinical Development, 20: 755-764. (co-first) IF=5.85

6. Rasouli, J., Casella, G., Ishikawa, L. L. W., Thome, R., Boehm, A., Ertel, A., Melo-Silva, C. R., Mari, E. R., Porazzi, P.,Zhang, W., Xiao, D., Sigal, L. J., Fortina, P., Zhang, G. X., Rostami, A.,Ciric, B. (2021) IFN-beta Acts on Monocytes to Ameliorate CNS Autoimmunity by Inhibiting Proinflammatory Cross-Talk Between Monocytes and Th Cells.Front Immunol, 12, 679498. IF=7.97

7. Rasouli, J., Casella, G., Yoshimura, S., Zhang, W., Xiao, D., Garifallou, J., Gonzalez, M.V., Wiedeman, A., Kus, A., Mari, E.R., Fortina, P., Hakonarson, H., Long, S.A., Zhang, G.X., Ciric, B., Rostami, A. (2020) A distinct GM-CSF(+) T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation. Sci Immunol, 5. IF=30.63

8. Li, X., Zhang, Y., Zhang, W., Xiao, D., Ciric, B., Rostami, A., Zhang, G.X. (2020) P7C3 attenuates CNS autoimmunity by inhibiting Th17 cell differentiation. Cell Mol Immunol, 10.1038/s41423-020-0497-5. IF=22.09

9. Casella, G., Rasouli, J., Thome, R., Descamps, H.C., Vattikonda, A., Ishikawa, L., Boehm, A., Hwang, D., Zhang, W., Xiao, D., Park, J., Zhang, G.-X., Alvarez, J.I., Rostami, A., Ciric, B. (2020) Interferon-γ/Interleukin-27 Axis Induces Programmed Death Ligand 1 Expression in Monocyte-Derived Dendritic Cells and Restores Immune Tolerance in Central Nervous System Autoimmunity. Front Immunol, 11, 2425. IF=7.97

10. Casella, G., Rasouli, J., Boehm, A., Zhang, W., Xiao, D., Ishikawa, L.L.W., Thome, R., Li, X., Hwang, D., Porazzi, P., Molugu, S., Tang, H.-Y., Zhang, G.-X., Ciric, B., Rostami, A. (2020) Oligodendrocyte-derived extracellular vesicles as antigen-specific therapy for autoimmune neuroinflammation in mice. Science Translational Medicine, 12, eaba0599. IF=19.32

11. Li, Y., Zhang, W., Zhao, J., Li, S., Shan, L., Zhu, J., Li, Y., Zhu, H., Mao, Q., Xia, H. (2018) Establishing a dual knock-out cell line by lentivirus based combined CRISPR/Cas9 and Loxp/Cre system. Cytotechnology, 70, 1595-1605. IF=2.0

12. Zhang, W., Xiao, D., Shan, L., Zhao, J., Mao, Q., Xia, H. (2017) Generation of apoptosis-resistant HEK293 cells with CRISPR/Cas mediated quadruple gene knockout for improved protein and virus production. Biotechnol Bioeng, 114, 2539-2549. IF=4.5

13. Xiao, D.#, Zhang, W.#, Li, Y., Liu, K., Zhao, J., Sun, X., Shan, L., Mao, Q., Xia, H. (2016) A novel luciferase knock-in reporter system for studying transcriptional regulation of the human Sox2 gene. J Biotechnol, 219, 110-116. (co-first author) IF=3.503

14. Chen, F., Zhang, W., Zhao, J., Yang, P., Ma, R., Xia, H. (2016) [Construction of Rev-erbbeta gene knockout HEK293 cell line with CRISPR/Cas9 system]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 32, 1446-1452.

15. Zhang, W., Wang, D., Liu, S., Zheng, X., Ji, H., Xia, H., Mao, Q. (2014) Multiple copies of a linear donor fragment released in situ from a vector improve the efficiency of zinc-finger nuclease-mediated genome editing. Gene Ther, 21, 282-288. IF=5.2

16. Zhang, W., Chen, H., Zheng, X., Wang, D., Ji, H., Xia, H., Mao, Q. (2014) Targeted genome correction by a single adenoviral vector simultaneously carrying an inducible zinc finger nuclease and a donor template. J Biotechnol, 188, 1-6. IF=3.503

17. Zhang, W., Zheng, X., Wang, Y., Mao, Q., Xia, H. (2012) Establishment of a cell line carrying single copy of an exogenous mutant reporter gene for assaying the biological activity of ZFNs. J Biotechnol, 162, 191-196. IF=3.503

18. Zhang, W., Guo, Y., Zhang, C., Ji, H., Meng, W., Wang, D., Li, X., Mao, Q., Xia, H. (2012) Rescue the failed half-ZFN by a sensitive mammalian cell-based luciferase reporter system. PLoS One, 7, e45169. IF=2.74

19. Li, X., Mao, Q., Wang, D., Zhang, W., Xia, H. (2012) A fiber chimeric CRAd vector Ad5/11-D24 double-armed with TRAIL and arresten for enhanced glioblastoma therapy. Hum Gene Ther, 23, 589-596. IF=5.7

20. Jiang, Y., Liu, J., Zhao, J., Zhang, W., Xia, H. (2010) Eukaryotic expression and identification of human Slit2 gene in HEK-293 cell line. Acta Universitatis Medicinalis Nanjing (Natural Science), 10.3724/SP.J.1238.2010.00512.

21. Liu, S., Mao, Q., Zhang, W., Zheng, X., Bian, Y., Wang, D., Li, H., Chai, L., Zhao, J., Xia, H. (2009) Genetically modified adenoviral vector with the protein transduction domain of Tat improves gene transfer to CAR-deficient cells. Biosci Rep, 29, 103-109. IF=2.942

22. Li, H., Zhang, W., Wang, D., Li, R., Qi, Z., Zhao, J., Xia, H. (2009) [Construction and expression of recombinant adenovirus vector carrying human endostatin, K5 and endostatin-K5 gene]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 25, 577-580.

23. Li, H., Zheng, X., Zhang, W., Bian, Y., Wang, D., Zhao, J., Xia, H. (2007) [Construction of an expression vector for high expression of siRNA]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 23, 1117-1118.

四、授权专利

1.一种抗肿瘤药物及其在脑胶质瘤治疗中的应用，中国，2024.02.13-2044.02.12，ZL 2023 10232504.5。

2.一种用于提高高容量腺病毒包装效率的辅助腺病毒，中国，2017.11.30-2037.11.29，ZL 2017 11239774.X。

3.一种抗凋亡细胞系及其建立方法和应用，2017.03.30-2037.03.29，中国，ZL 2017 10202547.3。

4.一种基因打靶系统，2015.6.10-2035.6.9，中国，ZL 2013 10157216.4。

5.锌指蛋白的快速筛选方法，2015.5.27-2035.5.26，中国，ZL 2012 1 0207952.1。

6.携带锌指核酸酶表达元件和供体DNA的腺病毒及其构建方法和应用，2014.12.10-2034.12.9，中国，ZL 2012 1 0447965.6。

7.快速检测锌指核酸酶介导基因定点整合的方法，2013.6.12-2033.6.11，中国，ZL 2011 1 0009773.2。

8.肿瘤特异嵌合启动子及其构建方法和应用，2011.11.30-2031.11.29，中国，ZL 2009 1 0023841.3。

9.表达小干扰RNA的VA1缺失的腺病毒载体及构建方法和应用，2011.5.4-2031.5.3，中国，ZL 2009 1 0022759.9。